Western blot analysis of TIM17 in extracts of various cell lines. Samples were incubated with TIM17 Polyclonal antibody (Product # PA5-92984) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry-Immunofluorescence analysis of TIM17 was performed in MCF7 cells using TIM17 Polyclonal Antibody (Product # PA5-92984).
Supplier Page from Thermo Fisher Scientific for TIM17 Antibody